LAB TOOL
Peptide reconstitution calculator
Work out the concentration of a reconstituted research vial. Enter the amount of peptide in the vial and the volume of diluent you add.
Stock concentration
5 mg/ml
- µg per ml
- 5,000
- Molar concentration
- -
- Select a compound
- Stock to add
- -
- Diluent to add
- -
Arithmetic only. Verify all preparations against your laboratory's validated procedures. Not for human or veterinary use.
How the calculation works
Concentration is the amount of peptide divided by the volume of diluent:
Multiply by 1,000 to convert mg/ml to µg/ml. Dividing mg/ml by the molecular weight (g/mol) gives the molar concentration. For a working dilution, the stock volume needed is C2 × V2 ÷ C1.
Read the full reconstitution guide for handling steps.
Need a diluent?
Calculator FAQs
How do I calculate the concentration of a reconstituted peptide?+
Divide the amount of peptide in the vial by the volume of diluent added. For example, 10mg in 2ml gives a stock concentration of 5mg/ml (5,000µg/ml).
Does adding more diluent change the amount of peptide in the vial?+
No. The total amount of peptide stays the same. More diluent lowers the concentration, so each measured volume contains less material.
How do I convert mg/ml to a molar concentration?+
Divide the mass concentration by the molecular weight. A concentration in mg/ml divided by the molecular weight in g/mol gives mol/l; multiply by 1,000,000 for µM. Select a compound above and the calculator uses its published molecular weight.
How do I make a working dilution from a stock solution?+
Use C1V1 = C2V2. The volume of stock needed is the target concentration multiplied by the final volume, divided by the stock concentration. Make up the remainder with diluent or assay buffer.
Which diluent should I use?+
Most laboratories use bacteriostatic water for multi-use reconstitution. Some compounds call for sterile water or dilute acetic acid; check the compound documentation and your own protocols.